Prime Minister Julia Gillard demanded on Tuesday that Pakistan investigate and explain the brutal killing of 21,000 Australian sheep in Karachi, a slaughter officials have described as appalling.
The shipment of Australian sheep was sent to the port city after being turned away by Bahrain, with Pakistan livestock officials ordering them to be culled over disease concerns.
Graphic footage of their slaughter was aired by ABC's Four Corners programme on Monday, including images of a man sawing at a sheep's neck before throwing it into a bloody trench.
Other sheep were bulldozed into the pit after being killed last month, but some were seen the next morning still breathing, sparking angry condemnation of their treatment.
Gillard said that she had spoken to her Raja Pervez Ashraf at a summit of European and Asian leaders in Laos to express her concern. "I did raise with the prime minister of Pakistan my concern about the graphic and very cruel images we've seen of the treatment of Australian sheep," she told reporters in Vientiane.
"I explained to him that Australians are distressed to see these acts of cruelty and that I wanted the matter investigated. "He undertook to investigate the matter ... I was very clear about Australia's concerns, very strong in raising those concerns and very clear that this is something that has distressed the Australian people."
Australia Agriculture Minister Joe Ludwig earlier called the slaughter "appalling" and his department disputed that the sheep were unhealthy. "We do not know the reasoning behind the Pakistan authority's decision to cull the sheep in Pakistan or their choice of the method used," the agriculture ministry said.
"We continue to hold that both the decision and the method used were unnecessary." "As far as we know the culling was done in accordance with the rules. The animals were slaughtered in Islamic manner," a senior official with Sindh livestock ministry said. "However, we will investigate the reports alleging the Australian sheep were brutalised during the slaughter."
Australia's National Farmers Federation said "decisive action" had been taken to temporarily suspend exports of sheep to Pakistan and Bahrain while investigations were carried out.