The epicentre of the 4.8-magnitude quake, which occurred at 0951 GMT, was 71 kilometres (44 miles) off the coast of Karpathos at a depth of around 10 kilometres, the institute said.
It came just three days after a 5.2-magnitude quake on the island of Crete and just under a week after a 4.2-magnitude tremor some 20 kilometres northwest of Athens.
Greece lies on major fault lines and is regularly hit by earthquakes, but they rarely cause casualties.
In 2017, a 6.7-magnitude earthquake killed two people on the island of Kos in the Aegean sea, causing significant damage.
In 1999, a 5.9-magnitude quake left 143 people dead in Athens and the region northwest of the capital.