The S&P 500 lost $2.138 trillion in market capitalization over the last four sessions, according to S&P Dow Jones Indices analyst Howard Silverblatt.
Fears of a pandemic escalated after the coronavirus spread to Spain and dozens of countries, from South Korea to Italy, accelerated emergency measures while Iran's virus death toll rose to 16, the highest outside China.
In the United States, the Centers for Disease Control and Prevention said Americans should prepare for possible community spread of the virus.
"The market's realizing that though the pace of the infections looked like it was slowing, it's still spreading globally," said Shawn Cruz, manager of trader strategy at TD Ameritrade in Jersey City, New Jersey.
While investors had hoped the economic impact of the virus would be contained to the first quarter, Cruz said many are now estimating that "it's going to have an impact on the first half of 2020 and probably beyond."
The Nasdaq ended the session 8.7% below its record closing high, reached last Wednesday, while the S&P finished 7.6% under its record close achieved the same day. A total 314 of the benchmark's 500 stocks were in correction territory, traditionally viewed as a 10% drop from their high.
The Dow ended the day 8.4% below its February 12 record close.
In the busiest trading day since December 21, 2018, volume on US exchanges was 12.24 billion shares, compared with the 7.99 billion average for the full session over the last 20 trading days.
The Dow Jones Industrial Average ended down 879.44 points, or 3.15%, at 27,081.36 and the S&P 500 lost 97.68 points, or 3.03%, to finish at 3,128.21. The Nasdaq Composite dropped 255.67 points, or 2.77%, to 8,965.61.
The Cboe Volatility Index, known as Wall Street's fear gauge, climbed above 30 for the first time since December 2018 and closed at 27.85.
The NYSE Arca Airline index closed down 5%, clocking its biggest three-day decline since October 2011. Delta Airlines Inc , down 6%, said on Tuesday that it expects US-China flights to be suspended until the end of April and expanded its travel waiver to Seoul until April 30.
The Associated Press reported that a senior member of the International Olympic Committee said organizers are more likely to cancel the 2020 Olympics than to postpone or move them if the coronavirus makes it too dangerous to hold in Tokyo.
Only 10 S&P stocks advanced on the day, while all of the S&P's 11 industry sectors fell. The energy sector was the biggest loser, with a more than 4% dip as oil prices tumbled.
Marriott International was the S&P's biggest percentage decliner, down almost 8%, and other travel stocks such as Tripadvisor, down 4.7%, and Norwegian Cruise Line Holdings, down 7.7%, also underperformed sharply.
Mastercard Inc shares fell 6.7%, also putting it among the S&P's biggest percentage decliners.
HP Inc, the S&P's biggest boost, pared early gains but still closed up 5.7% after saying it would step up efforts to slash costs and buy back stock as it sought investor support to defend against a $35 billion takeover offer from US printer maker Xerox Holdings Corp.
Declining issues outnumbered advancing ones on the NYSE by a 7.42-to-1 ratio; on Nasdaq, a 6.24-to-1 ratio favoured decliners. The S&P 500 posted four new 52-week highs and 43 new lows; the Nasdaq Composite recorded 28 new highs and 230 new lows.