Since the signing in Doha however, the militants have ramped up violence against Afghan forces, ending a partial truce and casting a pall over peace talks between Kabul and the Taliban, due to begin on March 10.
US Forces-Afghanistan spokesman Sonny Leggett tweeted that the airstrike targeted Taliban fighters who were "actively attacking" an Afghan forces checkpoint in Helmand.
"This was a defensive strike to disrupt the attack," he tweeted. "We call on the Taliban to stop needless attacks and uphold their commitments. As we have demonstrated, we will defend our partners when required."
He said insurgents had carried out 43 attacks on checkpoints in Helmand on Tuesday alone. Provincial police spokesman Mohammad Zaman Hamdard told AFP: "In the past two days we have witnessed the most intense Taliban attacks in Helmand."
"They have attacked several districts and many military bases," he added. Elsewhere the insurgents killed at least 20 Afghan soldiers and policemen in a series of overnight attacks in other provinces, government officials told AFP Wednesday.
"Taliban fighters attacked at least three army outposts in Imam Sahib district of Kunduz last night, killing at least 10 soldiers and four police," said Safiullah Amiri, a member of the provincial council. A defence ministry official speaking to AFP on condition of anonymity confirmed the army toll, while the provincial police spokesman Hejratullah Akbari confirmed the police fatalities.